Diagnosis of Brazilian vesiculoviruses by reverse transcription-polymerase chain reaction
نویسندگان
چکیده
منابع مشابه
Diagnosis of Brazilian vesiculoviruses by reverse transcription-polymerase chain reaction.
We describe a reverse transcription-polymerase chain reaction (RT-PCR) and a nested-PCR for diagnosis of Piry, Carajás, Cocal, and Alagoas vesiculoviruses from Brazil. The RNA extracts of viral and clinical samples were submitted to a RT-PCR using Vesiculovirus G primers that amplify part of the glycoprotein gene. The RT-PCR produced amplicons of expected size, 290 base pair, for the four studi...
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ژورنال
عنوان ژورنال: Memórias do Instituto Oswaldo Cruz
سال: 2005
ISSN: 0074-0276
DOI: 10.1590/s0074-02762005000200014